Widefield (epifluorescence)

DANTE 2s - Olympus IX81 widefield (Porter B047A)

Light Source & Filters

Light Sources:

  • Excelitas X-Cite XylisÌý365nm, modelÌýXT720S. The . Super high-intensity capable LED, intensity selectable in 1% increments, 5-100%. Excitation bandwidth from UV (365nm)Ìýto Near-IR (770nm) with peaks atÌý365, 430, 475, 545, 650, and 735nm, matching optimally with common fluorophores.
  • Halogen for brightfield and phase-contrast.

Standard filter configurations:Ìý

This system utilizes the finest optical filters from Semrock and Chroma and employs separate excitation and emission wheels for maximum flexibility and customizableÌýoptical settings. This system employsÌýfluor-matched single dichroic beamsplitters, and GFP/RFP dual and Ìýbeam splitters.ÌýThese components together provide maximum brightness and fast switching between wavelengths to provide the best results.Ìý

  • DAPI, Hoechst, Alexa350, 405, etc.
  • CFP, cerulean, etc.
  • Alexa488, FITC, EGFP, etc.
  • YFP, Venus, etc.
  • Alexa555, 568, 594, TxRed, mRFP, mCherry, TdTomato, etc.
  • Alexa647, Cy5, etc.
  • Alexa680/700/750, Cy7, etc.
  • Brightfield & Phase-contrast (PH2 20x andÌý40x objectives)

(These are common molecules imaged, for exact excitation and emission wavelengths please contact the Director)

Standard objectives:

  • 4x 0.13NA UPlanFl N UIS2 WD 17 (mm)
  • 10x 0.40NA UPlanApo Ph1 WD 3.1 (mm)
  • 20x 0.70NAÌýUPlanApo Ph2 WD 0.65 (mm)
  • 40x 0.75NA UPlanFl N Ph2 WD 0.51 (mm)
  • 60x 1.35NA UPlanSapo OIL WD 0.15 (mm)
  • 100x 1.40NAÌýUPlanSapo OIL WD 0.13 (mm)

Available upon request:Ìý

  • 60X 1.20NAÌýUPLSAPO WATER WD 0.28 (mm)
  • . 12/16 bit
  • 1344 x 1024, 6.45 x 6.45 micron pixels
  • binning, 2x2, 4x4, 8x8
  • peak QE >70%
  • wavelength range >30% QE, 360 - 800nm (low light mode)

High-speed large area montaging via , with advanced stitching in up to 7 wavelengths (inquire). Slide scanning for up toÌý4 standard 25x75mmÌýslides per run;Ìýentirety ofÌýslide(s), multiple areas across all slides,ÌýviaÌýPrior stage insert. IncludesÌýpre-defined settings for 1, 2, 3, or 4 slides in diffferent orientations. Pre-defined slide scanning or largeÌý50x75mm slide format,Ìývia Prior stage insert.

Stage and well-plate navigator interface withÌýpre-programmed plate formats for 6, 12, 24, 48, 96 wells and more.ÌýAdvanced 2D and 3D deconvolution;ÌýExperiment design GUI; Debluring;ÌýFluorescence unmixing; Image analysis;ÌýHigh dynamic range imaging (HDRI); more!!

Save your own custom settings including optical configurations, scan areas, acquisition settings,Ìýfor routine precision.

Live cell imaging with InVivo Sci. stage-top humidified chamber, ; 1x35mm, multi-well plates, pre-mixed 5% CO2, balance air (please contact Dr. Orth); Oustanding phase contrast (20x and 40x); precise repeatability over time viaÌýÌýZ-drive; FRET (CFP --> YFP);ÌýGFP/mCherry dual dichroic or Blue/Green/Red/FarRed/Near-IR penta beam splitters provide for rapid multicolor live sample imaging.

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Contact Director

Dante

PEPPER - Nikon TiU widefield with RGB color brightfield (Porter B047A)

Light Source & Filters

Light Sources:

  • Halogen illumination for brightfield and DIC

Standard filter configurations:

Semrock BrightLine quad Sedat
  • DAPI, Hoechst, etc.
  • Alexa488, FITC, GFP, etc.
  • TRITC, Alexa555, 568, 594, mRFP, mCherry, TdTomato, etc.
  • Alexa647, Cy5, etc.

Standard objectives:

  • 2x 0.10NA Plan Apo WD 8.5Ìý(mm)Ìý
  • 4x 0.13NA Plan FluorÌýWD 17.2 (mm)
  • 10x 0.45NA Plan Apo DIC N1ÌýWD 4 (mm)
  • 20x 0.50NA Plan FluorÌýWD 2.1 (mm)
  • 40xÌý0.60NA Plan FluorÌýWD 1.8Ìý(mm)
  • 100x 1.30NA Plan Fluor Ph3 OIL WD 0.20 (mm)

Optional objectives:

  • 60x 1.40NA Plan Apo VC λ OIL WD 0.13 (mm)
  • More (please inquire)
  • Ìýfor fluorescence
  • Ìýfor color brightfield (e.g. eosin, hematoxylin, crystal violet. etc.). This camera uses a CMOS chip with 5.9 megapixels.Ìý

Ìýmotorized stage. Sample holders for standard slide, 35 and 60 mm dishes, and multiwell plates. DIC possible at 10x, andÌý60x.ÌýBrightfield color RGB imaging.ÌýMulti-point imaging.ÌýMontaging with stitching.ÌýLive cell imaging with TOKAI-HIT Cell Incubator; 1 X 35 (mm) dish or chamber slide (coverslip bottom).ÌýRapid 4 color imaging via quad Sedat filter set (Semrock BrightLine).

Pepper

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