/lab/aaron-whiteley/ en /lab/aaron-whiteley/2021/06/01/molecular-basis-cd-ntase-nucleotide-selection-cbass-anti-phage-defense <span>Molecular basis of CD-NTase nucleotide selection in CBASS anti-phage defense</span> <span><span>Anonymous (not verified)</span></span> <span><time datetime="2021-06-01T00:00:00-06:00" title="Tuesday, June 1, 2021 - 00:00">Tue, 06/01/2021 - 00:00</time> </span> <div> <div class="imageMediaStyle focal_image_wide"> <img loading="lazy" src="/lab/aaron-whiteley/sites/default/files/styles/focal_image_wide/public/article-thumbnail/fx1_lrg_1.jpg?h=b1b266f7&amp;itok=P8q6QrWj" width="1200" height="800" alt="Graphical Abstract"> </div> </div> <div role="contentinfo" class="container ucb-article-tags" itemprop="keywords"> <span class="visually-hidden">Tags:</span> <div class="ucb-article-tag-icon" aria-hidden="true"> <i class="fa-solid fa-tags"></i> </div> <a href="/lab/aaron-whiteley/taxonomy/term/285" hreflang="en">CBASS</a> <a href="/lab/aaron-whiteley/taxonomy/term/286" hreflang="en">anti-phage</a> <a href="/lab/aaron-whiteley/taxonomy/term/219" hreflang="en">nucleotide second messenger</a> </div> <span>Govande AA</span> <span>,&nbsp;</span> <span>Duncan-Lowey B</span> <span>,&nbsp;</span> <span>Eaglesham JB</span> <span>,&nbsp;</span> <a href="/lab/aaron-whiteley/aaron-whiteley">Aaron Whiteley</a> <span>,&nbsp;</span> <span>Kranzusch PJ</span> <div class="ucb-article-content ucb-striped-content"> <div class="container"> <div class="paragraph paragraph--type--article-content paragraph--view-mode--default 3"> <div class="ucb-article-row-subrow row"> <div class="ucb-article-text col-lg d-flex align-items-center" itemprop="articleBody"> <div><p><em>Cell Reports</em> (2021) <span>PubMed PMID: </span><a href="https://www.ncbi.nlm.nih.gov/pubmed/34077735/" rel="nofollow"><span>34077735</span></a></p><h2>Abstract</h2><p>cGAS/DncV-like nucleotidyltransferase (CD-NTase) enzymes are signaling proteins that initiate antiviral immunity in animal cells and cyclic-oligonucleotide-based anti-phage signaling system (CBASS) phage defense in bacteria. Upon phage recognition, bacterial CD-NTases catalyze synthesis of cyclic-oligonucleotide signals, which activate downstream effectors and execute cell death. How CD-NTases control nucleotide selection to specifically induce defense remains poorly defined. Here, we combine structural and nucleotide-analog interference-mapping approaches to identify molecular rules controlling CD-NTase specificity. Structures of the cyclic trinucleotide synthase Enterobacter cloacae CdnD reveal coordinating nucleotide interactions and a possible role for inverted nucleobase positioning during product synthesis. We demonstrate that correct nucleotide selection in the CD-NTase donor pocket results in the formation of a thermostable-protein-nucleotide complex, and we extend our analysis to establish specific patterns governing selectivity for each of the major bacterial CD-NTase clades A-H. Our results explain CD-NTase specificity and enable predictions of nucleotide second-messenger signals within diverse antiviral systems.</p><h2>Links&nbsp;</h2><ul><li>DOI:&nbsp;<a href="https://doi.org/10.1016/j.celrep.2021.109206" rel="nofollow">10.1016/j.celrep.2021.109206</a></li><li>Journal Link: <a href="https://www.sciencedirect.com/science/article/pii/S2211124721005556" rel="nofollow">https://www.sciencedirect.com/science/article/pii/S2211124721005556</a></li></ul><h2>Citation</h2><p><span>Govande AA, Duncan-Lowey B, Eaglesham JB, Whiteley AT, Kranzusch PJ. </span><a href="https://www.ncbi.nlm.nih.gov/pubmed/34077735/" rel="nofollow">Molecular basis of CD-NTase nucleotide selection in CBASS anti-phage defense. </a><span>Cell Rep. 2021 Jun 1;35(9):109206. doi: 10.1016/j.celrep.2021.109206. PubMed PMID: 34077735.</span></p></div> </div> <div class="ucb-article-content-media ucb-article-content-media-right col-lg"> <div> <div class="paragraph paragraph--type--media paragraph--view-mode--default"> <div> <div class="imageMediaStyle large_image_style"> <img loading="lazy" src="/lab/aaron-whiteley/sites/default/files/styles/large_image_style/public/article-image/fx1_lrg_0.jpg?itok=2NQHCrbN" width="1500" height="1500" alt="Graphical Abstract"> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div>Govande AA, Duncan-Lowey B, Eaglesham JB, 鉃hiteley AT, Kranzusch PJ. | Cell Reports 2021</div> <h2> <div class="paragraph paragraph--type--ucb-related-articles-block paragraph--view-mode--default"> <div>Off</div> </div> </h2> <div>Traditional</div> <div>0</div> <div>On</div> <div>White</div> Tue, 01 Jun 2021 06:00:00 +0000 Anonymous 151 at /lab/aaron-whiteley